Interleukin 2 Induces both, Growth and Maturation of Lectin Reactive LYT-2+ but not LYT-2- Precursor Cells and Regulates the Cytolytic Potential of Effector Cells

Ute Hochgeschwender, T. Diamantstein, M. Prester, Gaby Nerz, M. M. Simon

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

This study investigated the requirements for lymphokines derived by recombinant (rec.) DNA technology for the induction of growth and maturation in highly purified lectin reactive T cell subsets. Nylon purified C57BL/6 lymph node T cells were treated with monoclonal anti-Lyt-2.2 or anti-L3T4 antibodies and fluorescence labeled (FITC) anti- immunoglobulin antibodies and were positively selected into Lyt-2+ (L3T4-) and Lyt-2- (L3T4+) lymphocyte subsets using a fluorescence-activated cell sorter. Sorted T lymphocytes, which were devoid of accessory cells were incubated either in bulk culture (2 x 102 - 3 x 104 cells/microculture) or under limiting dilution conditions (2.5–1000 cells/well) with lectin (Concanavalin A, Leukoagglutinin) and rec. human Interleukin 2 (rec. hIL-2) and/or rec. mouse Interferon Y (rec. mIFN-γ). The data show that Lyt-2+ lymphocytes respond to lectin and rec. hIL-2 with growth and development of cytolytic activity in the absence of other exogenous factor(s) or accessory cells. The presence of monoclonal antibodies to the Interleukin 2 receptor during the sensitization phase ablated the induction of Con A reactive precursor cells of cytolytic lymphocytes (CTL-P) by either rec. hIL-2 or conventional IL-2 containing lymphokine sources, indicating the essential role of IL-2 during activation of Lyt-2+ T lymphocytes. In contrast, Lyt-2- lymphocytes could not be induced by lectin and rec. hIL-2 alone for proliferation and always required the presence of accessory cells for significant growth. Exogenous rec. m IFNy was unable to induce growth and cytolytic activity in Con A reactive Lyt-2+ cells and did not significantly effect their response to rec. hIL-2. Limiting dilution experiments revealed that 10–16 % of the Lyt-2+ lymphocytes responded to Con A and rec.

Original languageEnglish
Pages (from-to)274-301
Number of pages28
JournalImmunobiology
Volume171
Issue number3
DOIs
StatePublished - 1986

Keywords

  • AC
  • B6
  • C57BL/6 mouse strain
  • CTL
  • CTL-P
  • Con A
  • ConASN
  • Concanavalin A
  • FCF
  • FCS
  • FITC
  • GTL-P
  • HYBSN
  • IFN-γ
  • IL-2
  • IL-2R
  • Interferon gamma
  • Interleukin 2
  • LA
  • LD
  • LN
  • Leukoagglutinin
  • MLC
  • MLCSN
  • PBS
  • PHA
  • PTL-P
  • Phytohemagglutinin
  • SC
  • SD
  • SN
  • T cell deprived, irradiated spleen cells
  • T,-P
  • [H]dThd
  • cytotoxic T lymphocytes
  • fetal calf serum
  • flow cytofluorometry
  • fluorescein isothiocyanate
  • irradiated whole spleen cells
  • limiting dilution
  • lymph node
  • mixed lymphocyte culture
  • phosphate-buffered saline
  • precursor cell of CTL
  • precursor cell of IFN-,γ-secreting T cell
  • precursor cell of growing T cell
  • precursor cell of proliferating T cell
  • rec. hIL-2
  • rec. mIFNγ
  • receptor for Interleukin 2
  • recombinant human Interleukin 2
  • recombinant mouse Interferon gamma
  • standard deviation
  • supernatant
  • supernatant from Con A-stimulated rat spleen cell
  • supernatant from hybridoma
  • supernatant from secondary mixed lymphocyte culture
  • tritiated thymidine

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