Intrastriatal iron perfusion releases dopamine: An in-vivo microdialysis study

A. Prikhojan, T. Brannan, M. D. Yahr

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We perfused iron as FeCl3 directly into the striatum of normal rats and used the in vivo microdialysis technique to monitor striatal levels of dopamine (DA). KCl was perfused to assess the functional integrity of the DA receptors at the end of each dialysis experiment. Cu+2 (as CuSO4) and Cl- (as NaCl) were perfused to compare the effects of Fe+3 to that of other heavy metal and donors of Cl- anion. Perfusion of FeCl3 (1mM for 15min) produced a 250% increase in striatal levels of DA. Perfusion of CuSO4 (1mM for 15min) or NaCl (10mM for 15min) did not affect striatal DA levels. There was a significant increase in DA levels with KCl stimulation (56mM for 15min) after perfusion with FeCl3. We conclude that iron releases DA from striatal nerve endings without the immediate destruction of the DA terminals. The implications of chronic release of dopamine as a cause of dopaminergic cell death are discussed.

Original languageEnglish
Pages (from-to)645-649
Number of pages5
JournalJournal of Neural Transmission
Volume109
Issue number5-6
DOIs
StatePublished - 2002

Keywords

  • Cerebral microdialysis
  • Dopamine
  • Iron
  • Parkinson's disease

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