Isolation and characterization of monoclonal antibodies against ribonuclease inhibitor

Mark Feldman, D. Stave Kohtz, David L. Kleinberg

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Mouse monoclonal antibodies were generated against human ribonuclease inhibitor, an intracellular regulatory protein. A total of four antibodies were isolated, all of which were of the immunoglobulin G1 subtype. Western blot analysis of the antibodies suggested monospecificity. Based on immunoradiometric competition assays two of the antibodies were determined to be directed against the same antigenic epitope, while the other two were against a second and possibly third epitope. None of the antibodies appeared to be directed against the ribonuclease binding site of the antigen. Data is presented suggesting that ribonuclease inhibitor is present in normal human serum. The potential significance of these findings is discussed.

Original languageEnglish
Pages (from-to)286-294
Number of pages9
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Nov 30 1988
Externally publishedYes


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