TY - JOUR
T1 - Long duration, room temperature preservation of filtered eDNA samples
AU - Wegleitner, Benjamin J.
AU - Jerde, Christopher L.
AU - Tucker, Andrew
AU - Chadderton, W. Lindsay
AU - Mahon, Andrew R.
N1 - Funding Information:
Thank you to B. Ridenhour and J. Jonas for assistance in sample collection. This project supported by the Great Lakes Restoration Initiative (F10AC00258) (#GL00E01102-0).
Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The use of environmental DNA is a rapidly evolving approach for surveillance and detection of species. Often water samples are collected in the field and then immediately cooled, filtered, and the resulting filters are stored in freezers to preserve the DNA for subsequent analyses. Recently it was shown that filtered samples could be stored at room temperature for 14 days without any discernable loss in the total DNA. However, for many conservation applications, particularly in remote settings with limited capacity to freeze samples, it would be advantageous to store samples at room temperature for longer periods of time. Here we test for significant loss of DNA yield from storage of polycarbonate track etched filters in Longmire’s lysis buffer at room temperature (20 °C) for 150 days.
AB - The use of environmental DNA is a rapidly evolving approach for surveillance and detection of species. Often water samples are collected in the field and then immediately cooled, filtered, and the resulting filters are stored in freezers to preserve the DNA for subsequent analyses. Recently it was shown that filtered samples could be stored at room temperature for 14 days without any discernable loss in the total DNA. However, for many conservation applications, particularly in remote settings with limited capacity to freeze samples, it would be advantageous to store samples at room temperature for longer periods of time. Here we test for significant loss of DNA yield from storage of polycarbonate track etched filters in Longmire’s lysis buffer at room temperature (20 °C) for 150 days.
KW - DNA
KW - Environmental DNA
KW - Methods
KW - ddPCR
UR - http://www.scopus.com/inward/record.url?scp=84947599841&partnerID=8YFLogxK
U2 - 10.1007/s12686-015-0483-x
DO - 10.1007/s12686-015-0483-x
M3 - Article
AN - SCOPUS:84947599841
VL - 7
SP - 789
EP - 791
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
SN - 1877-7252
IS - 4
ER -