Long duration, room temperature preservation of filtered eDNA samples

Benjamin J. Wegleitner, Christopher L. Jerde, Andrew Tucker, W. Lindsay Chadderton, Andrew R. Mahon

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The use of environmental DNA is a rapidly evolving approach for surveillance and detection of species. Often water samples are collected in the field and then immediately cooled, filtered, and the resulting filters are stored in freezers to preserve the DNA for subsequent analyses. Recently it was shown that filtered samples could be stored at room temperature for 14 days without any discernable loss in the total DNA. However, for many conservation applications, particularly in remote settings with limited capacity to freeze samples, it would be advantageous to store samples at room temperature for longer periods of time. Here we test for significant loss of DNA yield from storage of polycarbonate track etched filters in Longmire’s lysis buffer at room temperature (20 °C) for 150 days.

Original languageEnglish
Pages (from-to)789-791
Number of pages3
JournalConservation Genetics Resources
Volume7
Issue number4
DOIs
StatePublished - Dec 1 2015

Keywords

  • DNA
  • Environmental DNA
  • Methods
  • ddPCR

Fingerprint

Dive into the research topics of 'Long duration, room temperature preservation of filtered eDNA samples'. Together they form a unique fingerprint.

Cite this