Mannose-binding lectin without the aid of its associated serine proteases alters lipopolysaccharide-mediated cytokine/chemokine secretion from human endothelial cells

Hee Jung Kang, Sun Mi Lee, Hyeon Hwa Lee, Ji Yeon Kim, Byung Chul Lee, Jung Sun Yum, Hong Mo Moon, Bok Luel Lee

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Coupling between certain pathogen-associated molecular patterns and corresponding pattern recognition receptors of endothelial cells is important for the mediation of vascular inflammatory responses. Mannose-binding lectin (MBL) recognizes certain carbohydrate structures of microbes and subsequently activates the complement system as well as facilitates the phagocytosis of targets. We investigated whether MBL can intervene in the interaction between bacterial lipopolysaccharide (LPS) and endothelial cells to modulate subsequent inflammatory responses. In response to LPS, human umbilical vein endothelial cells (HUVEC) produced various cytokines/chemokines. Addition of the purified human MBL/MBL-associated serine proteases (MASP) complex or recombinant human MBL enhanced LPS-mediated cytokine/chemokine secretion by HUVEC, including interleukin-8 (IL-8), IL-6 and monocyte chemoattractant protein-1 in a dose-dependent manner. This enhancing effect was ameliorated by the addition of anti-MBL antibody or mannan. Among the cytokines/chemokines we analysed, IL-6 showed the greatest increase of secretion in the presence of native MBL/MASP complex or recombinant MBL. MBL, regardless of its association with MASP, alters LPS-mediated cytokine/chemokine secretion of HUVEC. Besides the well-known functions of MBL, to activate the lectin-complement pathway and to facilitate clearance of targets, alteration of cytokine/chemokine secretion may provide an additional role for MBL in modulating vascular inflammation.

Original languageEnglish
Pages (from-to)335-342
Number of pages8
JournalImmunology
Volume122
Issue number3
DOIs
StatePublished - Nov 2007
Externally publishedYes

Keywords

  • Chemokines/monokines
  • Endothelial cells
  • Interleukin-6
  • Lipopolysaccharide
  • Mannose-binding lectin

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