TY - JOUR
T1 - Molecular and evolutionary analysis of dengue virus serotype 2 isolates from Korean travelers in 2015
AU - Hwang, Eun Ha
AU - Kim, Green
AU - Oh, Hanseul
AU - An, You Jung
AU - Kim, Jiyeon
AU - Kim, Jung Heon
AU - Hwang, Eung Soo
AU - Park, Jong Hwan
AU - Hong, Jung Joo
AU - Koo, Bon Sang
N1 - Funding Information:
The pathogen resources (NCCP43248, NCCP43253 and NCCP43254) for this study were provided by the National Culture Collection for Pathogens.
Funding Information:
This work was supported by a grant from the KRIBB Research Initiative Program (KGM4571922), Republic of Korea. Acknowledgements
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/8/1
Y1 - 2020/8/1
N2 - In Korea, dengue infection has been frequently reported in travelers to tropical and subtropical countries. Global warming increases the probability of autochthonous dengue outbreaks in Korea. In this report, the molecular and evolutionary properties of four dengue virus (DENV) type 2 isolates from Korean overseas travelers were examined. Three of these isolates were classified as Cosmopolitan genotypes and further divided into sublineages 1 (43,253, 43,254) and 2 (43,248), while the other isolate (KBPV-VR29) was related to American genotypes. The variable amino acid motifs related to virulence and replication were identified in the structural and non-structural proteins. A negative selection mechanism was clearly verified in all of the DENV proteins. Potential recombination events were identified in the NS5 protein of the XSBN10 strain. The substitution rate (5.32 × 10−4 substitutions per site) and the time of the most recent common ancestor (TMRCA) for each evolutionary group were determined by the Bayesian skyline coalescent method. This study shows that DENV type 2 strains with distinct phylogenetic, evolutionary, and virulence characteristics have been introduced into Korea by overseas travelers and have the potential to trigger autochthonous dengue outbreaks.
AB - In Korea, dengue infection has been frequently reported in travelers to tropical and subtropical countries. Global warming increases the probability of autochthonous dengue outbreaks in Korea. In this report, the molecular and evolutionary properties of four dengue virus (DENV) type 2 isolates from Korean overseas travelers were examined. Three of these isolates were classified as Cosmopolitan genotypes and further divided into sublineages 1 (43,253, 43,254) and 2 (43,248), while the other isolate (KBPV-VR29) was related to American genotypes. The variable amino acid motifs related to virulence and replication were identified in the structural and non-structural proteins. A negative selection mechanism was clearly verified in all of the DENV proteins. Potential recombination events were identified in the NS5 protein of the XSBN10 strain. The substitution rate (5.32 × 10−4 substitutions per site) and the time of the most recent common ancestor (TMRCA) for each evolutionary group were determined by the Bayesian skyline coalescent method. This study shows that DENV type 2 strains with distinct phylogenetic, evolutionary, and virulence characteristics have been introduced into Korea by overseas travelers and have the potential to trigger autochthonous dengue outbreaks.
UR - http://www.scopus.com/inward/record.url?scp=85084804932&partnerID=8YFLogxK
U2 - 10.1007/s00705-020-04653-z
DO - 10.1007/s00705-020-04653-z
M3 - Article
C2 - 32409874
AN - SCOPUS:85084804932
VL - 165
SP - 1739
EP - 1748
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 8
ER -