New spectral imaging techniques for blood oximetry in the retina

Ied Alabboud, Gonzalo Muyo, Alistair Gorman, David Mordant, Andrew McNaught, Clement Petres, Yvan R. Petillot, Andrew R. Harvey

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

22 Scopus citations

Abstract

Hyperspectral imaging of the retina presents a unique opportunity for direct and quantitative mapping of retinal biochemistry - particularly of the vasculature where blood oximetry is enabled by the strong variation of absorption spectra with oxygenation. This is particularly pertinent both to research and to clinical investigation and diagnosis of retinal diseases such as diabetes, glaucoma and age-related macular degeneration. The optimal exploitation of hyperspectral imaging however, presents a set of challenging problems, including; the poorly characterised and controlled optical environment of structures within the retina to be imaged; the erratic motion of the eye ball; and the compounding effects of the optical sensitivity of the retina and the low numerical aperture of the eye. We have developed two spectral imaging techniques to address these issues. We describe first a system in which a liquid crystal tuneable filter is integrated into the illumination system of a conventional fundus camera to enable time-sequential, random access recording of narrow-band spectral images. Image processing techniques are described to eradicate the artefacts that may be introduced by time-sequential imaging. In addition we describe a unique snapshot spectral imaging technique dubbed IRIS that employs polarising interferometry and Wollaston prism beam splitters to simultaneously replicate and spectrally filter images of the retina into multiple spectral bands onto a single detector array. Results of early clinical trials acquired with these two techniques together with a physical model which enables oximetry map are reported.

Original languageEnglish
Title of host publicationNovel Optical Instrumentation for Biomedical Applications III
PublisherSPIE
ISBN (Print)0819467758, 9780819467751
DOIs
StatePublished - 2007
EventNovel Optical Instrumentation for Biomedical Applications III - Munich, Germany
Duration: Jun 17 2007Jun 19 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6631
ISSN (Print)1605-7422

Conference

ConferenceNovel Optical Instrumentation for Biomedical Applications III
Country/TerritoryGermany
CityMunich
Period06/17/0706/19/07

Keywords

  • Blood oximetry
  • Retinal imaging
  • Spectral imaging

Fingerprint

Dive into the research topics of 'New spectral imaging techniques for blood oximetry in the retina'. Together they form a unique fingerprint.

Cite this