Noninvasive Raman spectroscopy of rat tibiae: Approach to in vivo assessment of bone quality

Paul I. Okagbare; Dana Begun; Mary Tecklenburg; Ayorinde Awonusi; Steven A. Goldstein; Michael D. Morris

doi:10.1117/1.JBO.17.9.090502

Noninvasive Raman spectroscopy of rat tibiae: Approach to in vivo assessment of bone quality

Paul I. Okagbare, Dana Begun, Mary Tecklenburg, Ayorinde Awonusi, Steven A. Goldstein, Michael D. Morris

https://www.cmich.edu/academics/colleges/college-science-engineering/departments-schools/chemistry-and-biochemistry

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21 Scopus citations

Abstract

We report on in vivo noninvasive Raman spectroscopy of rat tibiae using robust fiber-optic Raman probes and holders designed for transcutaneous Raman measurements in small animals. The configuration allows placement of multiple fibers around a rat leg, maintaining contact with the skin. Bone Raman data are presented for three regions of the rat tibia diaphysis with different thicknesses of overlying soft tissue. The ability to perform in vivo noninvasive Raman measurement and evaluation of subtle changes in bone composition is demonstrated with rat leg phantoms in which the tibia has carbonated hydroxylapatite, with different carbonate contents. Our data provide proof of the principle that small changes in bone composition can be monitored through soft tissue at anatomical sites of interest in biomedical studies.

Original language	English
Article number	090502
Journal	Journal of Biomedical Optics
Volume	17
Issue number	9
DOIs	https://doi.org/10.1117/1.JBO.17.9.090502
State	Published - Sep 2012

Keywords

carbonated hydroxyapatite
fiber-optic probes
in-vivo Raman
rat tibia

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title = "Noninvasive Raman spectroscopy of rat tibiae: Approach to in vivo assessment of bone quality",

abstract = "We report on in vivo noninvasive Raman spectroscopy of rat tibiae using robust fiber-optic Raman probes and holders designed for transcutaneous Raman measurements in small animals. The configuration allows placement of multiple fibers around a rat leg, maintaining contact with the skin. Bone Raman data are presented for three regions of the rat tibia diaphysis with different thicknesses of overlying soft tissue. The ability to perform in vivo noninvasive Raman measurement and evaluation of subtle changes in bone composition is demonstrated with rat leg phantoms in which the tibia has carbonated hydroxylapatite, with different carbonate contents. Our data provide proof of the principle that small changes in bone composition can be monitored through soft tissue at anatomical sites of interest in biomedical studies.",

keywords = "carbonated hydroxyapatite, fiber-optic probes, in-vivo Raman, rat tibia",

author = "Okagbare, {Paul I.} and Dana Begun and Mary Tecklenburg and Ayorinde Awonusi and Goldstein, {Steven A.} and Morris, {Michael D.}",

note = "Funding Information: The authors acknowledge support of this work through National Institutes of Health (NIH) Grants R01AR055222 and R01AR056646. The authors also thank Kathleen Sweet for help with animal handling and Charles Roehm for construction of the optical fiber holder.",

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AU - Awonusi, Ayorinde

AU - Goldstein, Steven A.

AU - Morris, Michael D.

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Noninvasive Raman spectroscopy of rat tibiae: Approach to in vivo assessment of bone quality

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Keywords

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