Noninvasive Raman spectroscopy of rat tibiae: Approach to in vivo assessment of bone quality

Paul I. Okagbare, Dana Begun, Mary Tecklenburg, Ayorinde Awonusi, Steven A. Goldstein, Michael D. Morris

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


We report on in vivo noninvasive Raman spectroscopy of rat tibiae using robust fiber-optic Raman probes and holders designed for transcutaneous Raman measurements in small animals. The configuration allows placement of multiple fibers around a rat leg, maintaining contact with the skin. Bone Raman data are presented for three regions of the rat tibia diaphysis with different thicknesses of overlying soft tissue. The ability to perform in vivo noninvasive Raman measurement and evaluation of subtle changes in bone composition is demonstrated with rat leg phantoms in which the tibia has carbonated hydroxylapatite, with different carbonate contents. Our data provide proof of the principle that small changes in bone composition can be monitored through soft tissue at anatomical sites of interest in biomedical studies.

Original languageEnglish
Article number090502
JournalJournal of Biomedical Optics
Issue number9
StatePublished - Sep 2012


  • carbonated hydroxyapatite
  • fiber-optic probes
  • in-vivo Raman
  • rat tibia


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