TY - JOUR
T1 - Normal cellular prior protein is preferentially expressed on subpopulations of murine hemopoietic cells
AU - Liu, T.
AU - Li, R.
AU - Wong, B. S.
AU - Liu, D.
AU - Pan, T.
AU - Petersen, R. B.
AU - Gambetti, P.
AU - Sy, M. S.
PY - 2001/3/15
Y1 - 2001/3/15
N2 - We studied the expression of normal cellular prion protein (PrPC) in mouse lymphoid tissues with newly developed mAbs to PrPC. Most of the mature T and B cells in the peripheral lymphoid organs do not express PrPC. In contrast, most thymocytes are PrPC+. In the bone marrow, erythroid cells and maturing granulocytes are PrPC+. Approximately 50% of the cells in the region of small lymphocytes and progenitor cells also express PrPC. Most of these PrPC+ cells are CD43+, but B220-, surface IgM- (sIgM-), and IL-7R-, a phenotype that belongs to cells not yet committed to the B cell lineage. Another small group of the PrPC+ cell are B220+, and some of these are also sIgM+. The majority of the B220+ cells, however, are PrPC-. Therefore, PrPC is preferentially expressed in early bone marrow progenitor cells and subsets of maturing B cells. Supporting this interpretation is our observation that stimulation of bone marrow cells in vitro with PMA results in a decrease in the number of PrPC+B220- cells with a corresponding increase of sIgM+B220high mature B cells. This result suggests that the PrPC+B220- cells are potential progenitors. Furthermore, in the bone marrow of Rag-1-/- mice, there are an increased number of PrPC+B220- cells, and most of the developmentally arrested pro-B cells in these mice are PrPC+. Collectively, these results suggest that PrPC is expressed preferentially in immature T cells in the thymus and early progenitor cells in the bone marrow, and the expression of PrPC is regulated during hemopoietic differentiation.
AB - We studied the expression of normal cellular prion protein (PrPC) in mouse lymphoid tissues with newly developed mAbs to PrPC. Most of the mature T and B cells in the peripheral lymphoid organs do not express PrPC. In contrast, most thymocytes are PrPC+. In the bone marrow, erythroid cells and maturing granulocytes are PrPC+. Approximately 50% of the cells in the region of small lymphocytes and progenitor cells also express PrPC. Most of these PrPC+ cells are CD43+, but B220-, surface IgM- (sIgM-), and IL-7R-, a phenotype that belongs to cells not yet committed to the B cell lineage. Another small group of the PrPC+ cell are B220+, and some of these are also sIgM+. The majority of the B220+ cells, however, are PrPC-. Therefore, PrPC is preferentially expressed in early bone marrow progenitor cells and subsets of maturing B cells. Supporting this interpretation is our observation that stimulation of bone marrow cells in vitro with PMA results in a decrease in the number of PrPC+B220- cells with a corresponding increase of sIgM+B220high mature B cells. This result suggests that the PrPC+B220- cells are potential progenitors. Furthermore, in the bone marrow of Rag-1-/- mice, there are an increased number of PrPC+B220- cells, and most of the developmentally arrested pro-B cells in these mice are PrPC+. Collectively, these results suggest that PrPC is expressed preferentially in immature T cells in the thymus and early progenitor cells in the bone marrow, and the expression of PrPC is regulated during hemopoietic differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0035866560&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.166.6.3733
DO - 10.4049/jimmunol.166.6.3733
M3 - Article
C2 - 11238614
AN - SCOPUS:0035866560
VL - 166
SP - 3733
EP - 3742
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -