Novel luciferase–opsin combinations for improved luminopsins

Sung Young Park, Sang Ho Song, Brandon Palmateer, Akash Pal, Eric D. Petersen, Gabrielle P. Shall, Ryan M. Welchko, Keiji Ibata, Atsushi Miyawaki, George J. Augustine, Ute Hochgeschwender

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Previous work has demonstrated that fusion of a luciferase to an opsin, to create a luminescent opsin or luminopsin, provides a genetically encoded means of manipulating neuronal activity via both chemogenetic and optogenetic approaches. Here we have expanded and refined the versatility of luminopsin tools by fusing an alternative luciferase variant with high light emission, Gaussia luciferase mutant GLucM23, to depolarizing and hyperpolarizing channelrhodopsins with increased light sensitivity. The combination of GLucM23 with Volvox channelrhodopsin-1 produced LMO4, while combining GLucM23 with the anion channelrhodopsin iChloC yielded iLMO4. We found efficient activation of these channelrhodopsins in the presence of the luciferase substrate, as indicated by responses measured in both single neurons and in neuronal populations of mice and rats, as well as by changes in male rat behavior during amphetamine-induced rotations. We conclude that these new luminopsins will be useful for bimodal opto- and chemogenetic analyses of brain function.

Original languageEnglish
Pages (from-to)410-421
Number of pages12
JournalJournal of Neuroscience Research
Issue number3
StatePublished - Mar 1 2020


  • 6-OHDA rat
  • GLucM23
  • bioluminescence
  • chemogenetics
  • iChloC
  • optogenetics


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