A monoclonal antibody, A‐7C11, was generated which reacts with two polypeptides of 40 kDa and 80 kDa associated with the coat proteins of purified brain clathirn‐coated vesicles. The 40‐kDa antigen was purified and found to display actin‐binding properties. Negative‐staining electron microscopy showed that one of the antigens reactive with A‐7C11 appears to mediate the association of isolated clathrin‐coated vesicles with assembling actin filaments in vitro. Immunofluorescence microscopy of cultured fibroblasts with A‐7C11 revealed the antigens aligned with both actin filaments and as punctate structures near the plasma membrane. The data suggest that the interaction between clathrin‐coated vesicles and the actin cytoskeleton is mediated by antigens identified by monoclonal antibody A‐7C11.
|Number of pages||8|
|Journal||European Journal of Biochemistry|
|State||Published - Sep 1990|