Original artical Prediction of recurrence risk in early breast cancer using human epidermal growth factor 2 and cyclin A2

Hui Ping Li, Jia Fu Ji, Kuan Yong Hou, Yu Tao Lei, Hong Mei Zhao, Jing Wang, Jie Zheng, Jian Ying Liu, Mo Pei Wang, Yu Xiao, You Fan Wang, You Yong Lü, Yan Sun

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Background: Human epidermal growth factor 2 (HER2) is one of the most important prediction factors, but only 25%-30% of breast cancer patients HER2 are positive. It is unknown whether there are other molecular markers that could be used to predict prognosis and recurrence in HER2 negative patients.This study investigated correlations of cyclin A2 and HER2 levels with clinical outcomes in 281 patients with invasive breast cancer in order to identify whether cyclin A2 can serve as a prognostic factor in HER2 negative patients. Methods: Immunohistochemical staining was used to detect cyclin A2 and HER2 expression in 281 patients. Cyclin A2 and HER2 gene amplifications were analyzed using gene analysis and RT-PCR in 12 patients. Risk and survival estimates were analyzed using Log-rank, Kaplan-Meier, and Cox regression analysis; cyclin A2 and HER2 consistency with survival were analyzed using Kappa analysis. Results: Patients with higher cyclin A2 and HER2 expressions had significantly shorter disease-free survival periods (P=0.047 and P=0.05, respectively). Kappa analysis performed that cyclin A2 and HER2 showed a low Kappa index (kappa=0.37), allowing us to conclude that cyclin A2 and HER2 detect different pathologies. Gene analysis and RT-PCR showed that cyclin A2 was upregulated in patients with early relapse; the average increase was 3.69-2.74 fold. Conclusions: Cyclin A2 and HER2 are associated with proliferation and high recurrence, particularly when combined. Cyclin A2 is easily detected by nuclear staining and might be a useful biomarker for recurrence risk in HER2 negative patients.

Original languageEnglish
Pages (from-to)431-437
Number of pages7
JournalChinese Medical Journal
Issue number4
StatePublished - Feb 20 2010


  • Breast neoplasms
  • Cyclin A2
  • Human epidermal growth factor 2
  • Prognosis


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