TY - JOUR
T1 - Polyunsaturated fatty acids acutely suppress antibodies to malondialdehyde-modified lipoproteins in patients with vascular disease
AU - Gradek, W. Quinten
AU - Harris, Matthey T.
AU - Yahia, Najat
AU - Davis, Warren W.
AU - Le, Ngoc Anh
AU - Brown, W. Virgil
N1 - Funding Information:
Patients were given 4 test meals administered at least 1 week apart. All meals were prepared by the General Clinical Research Center (GCRC) kitchen, which is supported by grant M01RR00039 from the National Institutes of Health. All meals contained identical amounts of sucrose (45 g), nonfat yogurt (113 g), and frozen orange juice concentrate (36.2 g). The high carbohydrate/low fat meal contained only the preceding ingredients and no oil. The other 3 meals contained 50.1 g of coconut oil, canola oil, or safflower oil, respectively. Canola and safflower oils were selected to provide a high content of monounsaturated and polyunsaturated fats, respectively, while contributing minimal amounts of saturated fat to each meal (3.7 g canola oil and 4.7 g safflower oil). The macronutrients of the meals are listed in The percentage of saturated, monounsaturated, and polyunsaturated fatty acids in each oil is listed as provided by the supplier. The meals were coded by the GCRC kitchen and administered in a random order and double-blind manner. Table 2 .
PY - 2004/4/1
Y1 - 2004/4/1
N2 - Antibodies to malondialdehyde (MDA)-modified low-density lipoprotein (LDL) are transiently reduced during the postprandial period in patients with atherosclerotic heart disease. Previous experiments using mixed meals high in fat have found reductions of approximately 20% within 2 hours. This study was designed to reveal if this phenomenon was due uniquely to saturated fats. Using a standardized immunoassay for the detection of circulating levels of autoantibodies (AAbs) against MDA-modified LDL, we examined the acute changes in AAb levels during 6 hours of postprandial lipemia in 10 men with known atherosclerotic heart disease. Each subject was given 4 meals 1 week apart. Three of the meals differed in content of saturated fat, monounsaturated fat, and polyunsaturated fat. A fat-free meal with equivalent carbohydrate and equivalent protein was also administered. The study was double blinded and the meals were administered in random order. Only the meal enriched with polyunsaturated fatty acids caused the reduction in antibodies to MDA-LDL. This decrease was statistically significant from baseline at 1 hour (p <0.05), 2 hours (p <0.004), and 3 hours (p <0.02), with the nadir occurring at 2 hours. Polyunsaturated fatty acids are the major stimulus for causing a reduction in the AAbs to MDA-LDL during postprandial lipemia. These results strongly suggest that fat in chylomicrons may be a major source of oxidized lipids in the blood of patients with arteriosclerosis.
AB - Antibodies to malondialdehyde (MDA)-modified low-density lipoprotein (LDL) are transiently reduced during the postprandial period in patients with atherosclerotic heart disease. Previous experiments using mixed meals high in fat have found reductions of approximately 20% within 2 hours. This study was designed to reveal if this phenomenon was due uniquely to saturated fats. Using a standardized immunoassay for the detection of circulating levels of autoantibodies (AAbs) against MDA-modified LDL, we examined the acute changes in AAb levels during 6 hours of postprandial lipemia in 10 men with known atherosclerotic heart disease. Each subject was given 4 meals 1 week apart. Three of the meals differed in content of saturated fat, monounsaturated fat, and polyunsaturated fat. A fat-free meal with equivalent carbohydrate and equivalent protein was also administered. The study was double blinded and the meals were administered in random order. Only the meal enriched with polyunsaturated fatty acids caused the reduction in antibodies to MDA-LDL. This decrease was statistically significant from baseline at 1 hour (p <0.05), 2 hours (p <0.004), and 3 hours (p <0.02), with the nadir occurring at 2 hours. Polyunsaturated fatty acids are the major stimulus for causing a reduction in the AAbs to MDA-LDL during postprandial lipemia. These results strongly suggest that fat in chylomicrons may be a major source of oxidized lipids in the blood of patients with arteriosclerosis.
UR - http://www.scopus.com/inward/record.url?scp=1842425182&partnerID=8YFLogxK
U2 - 10.1016/j.amjcard.2003.12.028
DO - 10.1016/j.amjcard.2003.12.028
M3 - Article
C2 - 15050493
AN - SCOPUS:1842425182
SN - 0002-9149
VL - 93
SP - 881
EP - 885
JO - The American Journal of Cardiology
JF - The American Journal of Cardiology
IS - 7
ER -