TY - JOUR
T1 - Prestorage gamma irradiation induces oxidative injury to red cells
AU - Katharia, Rahul
AU - Chaudhary, Rajendra
AU - Agarwal, Prashant
N1 - Funding Information:
This research was supported by SAO grant GO3-4024X and by NASA/XMM grant NAG5-13266. E. B. was supported by the Yigal-Alon Fellowship, by ISF grant 28/03, and by a grant from the Asher Space Research Institute.
PY - 2013/2
Y1 - 2013/2
N2 - Background: Gamma irradiation of blood results in the formation free radicals, which interact with lipids and proteins in the membranes of red blood cells. We have investigated oxidative injury to gamma-irradiated red cells by measuring markers of oxidative injury and its correlation with red cell membrane damage. Methods: Thirty red cell blood units were irradiated at 25Gy using Gamma Irradiator (Nordion, Canada) and stored at 4°C for 28days. Markers of oxidative injury such as MDA levels, methemoglobin formation and osmotic fragility and markers of membrane damage including supernatant Hb, supernatant K+, and LDH were studied. Results: There was a progressive and statistically significant increase in markers of oxidative injury such as MDA (3.76 v/s 5.01), and methemoglobin formation (1.87 v/s 3.58) in irradiated red cells compared to control non-irradiated cells. Exposure to gamma irradiation caused significant increase in markers of hemolysis such as supernatant Hb (0.087 v/s 0.363), K+ (35.1 v/s 51.2) and LDH (366.9 v/s 587.4) over the storage period of 28days. Conclusion: Gamma irradiation increases lipid peroxidation and oxidative injury to the red cells.
AB - Background: Gamma irradiation of blood results in the formation free radicals, which interact with lipids and proteins in the membranes of red blood cells. We have investigated oxidative injury to gamma-irradiated red cells by measuring markers of oxidative injury and its correlation with red cell membrane damage. Methods: Thirty red cell blood units were irradiated at 25Gy using Gamma Irradiator (Nordion, Canada) and stored at 4°C for 28days. Markers of oxidative injury such as MDA levels, methemoglobin formation and osmotic fragility and markers of membrane damage including supernatant Hb, supernatant K+, and LDH were studied. Results: There was a progressive and statistically significant increase in markers of oxidative injury such as MDA (3.76 v/s 5.01), and methemoglobin formation (1.87 v/s 3.58) in irradiated red cells compared to control non-irradiated cells. Exposure to gamma irradiation caused significant increase in markers of hemolysis such as supernatant Hb (0.087 v/s 0.363), K+ (35.1 v/s 51.2) and LDH (366.9 v/s 587.4) over the storage period of 28days. Conclusion: Gamma irradiation increases lipid peroxidation and oxidative injury to the red cells.
KW - Gamma irradiation
KW - Lipid peroxidation
KW - Oxidative injury
KW - Red cells
UR - http://www.scopus.com/inward/record.url?scp=84873137536&partnerID=8YFLogxK
U2 - 10.1016/j.transci.2012.07.004
DO - 10.1016/j.transci.2012.07.004
M3 - Article
C2 - 22939969
AN - SCOPUS:84873137536
SN - 1473-0502
VL - 48
SP - 39
EP - 43
JO - Transfusion and Apheresis Science
JF - Transfusion and Apheresis Science
IS - 1
ER -