Prestorage gamma irradiation induces oxidative injury to red cells

Rahul Katharia, Rajendra Chaudhary, Prashant Agarwal

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Background: Gamma irradiation of blood results in the formation free radicals, which interact with lipids and proteins in the membranes of red blood cells. We have investigated oxidative injury to gamma-irradiated red cells by measuring markers of oxidative injury and its correlation with red cell membrane damage. Methods: Thirty red cell blood units were irradiated at 25Gy using Gamma Irradiator (Nordion, Canada) and stored at 4°C for 28days. Markers of oxidative injury such as MDA levels, methemoglobin formation and osmotic fragility and markers of membrane damage including supernatant Hb, supernatant K+, and LDH were studied. Results: There was a progressive and statistically significant increase in markers of oxidative injury such as MDA (3.76 v/s 5.01), and methemoglobin formation (1.87 v/s 3.58) in irradiated red cells compared to control non-irradiated cells. Exposure to gamma irradiation caused significant increase in markers of hemolysis such as supernatant Hb (0.087 v/s 0.363), K+ (35.1 v/s 51.2) and LDH (366.9 v/s 587.4) over the storage period of 28days. Conclusion: Gamma irradiation increases lipid peroxidation and oxidative injury to the red cells.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalTransfusion and Apheresis Science
Issue number1
StatePublished - Feb 2013
Externally publishedYes


  • Gamma irradiation
  • Lipid peroxidation
  • Oxidative injury
  • Red cells


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