TY - JOUR
T1 - Prion protein expression in different species
T2 - Analysis with a panel of new mAbs
AU - Zanusso, Gianluigi
AU - Liu, Dacai
AU - Ferrari, Sergio
AU - Hegyi, Ivan
AU - Yin, Xinghua
AU - Aguzzi, Adriano
AU - Hornemann, Simone
AU - Liemann, Susanne
AU - Glockshuber, Rudi
AU - Manson, Jean C.
AU - Brown, Paul
AU - Petersen, Robert B.
AU - Gambetti, Pierluigi
AU - Sy, Man Sun
PY - 1998/7/21
Y1 - 1998/7/21
N2 - By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrP(c)), we obtained a panel of mAbs specific for murine PrP(c). These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrP(c)) and protease- resistant (PrP(res)) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPw(c). These newly generated PrP(c) antibodies will help to explore the biology of PrP(c) and to establish the diagnosis of prion diseases in both humans and animals.
AB - By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrP(c)), we obtained a panel of mAbs specific for murine PrP(c). These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrP(c)) and protease- resistant (PrP(res)) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPw(c). These newly generated PrP(c) antibodies will help to explore the biology of PrP(c) and to establish the diagnosis of prion diseases in both humans and animals.
UR - http://www.scopus.com/inward/record.url?scp=13144256747&partnerID=8YFLogxK
U2 - 10.1073/pnas.95.15.8812
DO - 10.1073/pnas.95.15.8812
M3 - Article
C2 - 9671761
AN - SCOPUS:13144256747
VL - 95
SP - 8812
EP - 8816
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 15
ER -