Probing the mycobacterial trehalome with bioorthogonal chemistry

Cynthia M. Holsclaw, Benjamin Swarts

Research output: Contribution to journalArticlepeer-review

Abstract

Mycobacteria, including the pathogen Mycobacterium tuberculosis, use the non-mammalian disaccharide trehalose as a precursor for essential cell-wall glycolipids and other metabolites. Here we describe a strategy for exploiting trehalose metabolic pathways to label glycolipids in mycobacteria with azide-modified trehalose (TreAz) analogues. Subsequent bioorthogonal ligation with alkyne-functionalized probes enabled detection and visualization of cell-surface glycolipids. Characterization of the metabolic fates of four TreAz analogues revealed unique labeling routes that can be harnessed for pathway-targeted investigation of the mycobacterial trehalome.
Original languageEnglish
Pages (from-to)16123--16126
JournalJ. Am. Chem. Soc.
Volume134
Issue number39
StatePublished - 2012

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