TY - JOUR
T1 - Proteomic characterization of differentially expressed proteins associated with no stress in retinal ganglion cells
AU - Kim, Jum Ji
AU - Kim, Yeon Hyang
AU - Lee, Mi Young
PY - 2009/7
Y1 - 2009/7
N2 - Proteomic analyses of differentially expressed proteins in rat retinal ganglion cells (RGC-5) following S-nitrosoglutathione (GSNO), an NO donor, treatment were conducted. Of the approximately 314 protein spots that were detected, 19 were differentially expressed in response to treatment with GSNO. Of these, 14 proteins were up-regulated and 5 were down-regulated. Notably, an increase in GAPDH expression following GSNO treatment was detected in RGC-5 cells through Western blotting as well as proteomics. The increased GAPDH expression in response to GSNO treatment was accompanied by an increase in Herc6 protein, an E3 ubiquitin ligase. Moreover, GSNO treatment resulted in the translocation of GADPH from the cytosol to the nucleus and its subsequent accumulation. These results suggest that NO stress-induced apoptosis may be associated with the nuclear translocation and accumulation of GAPDH in RGC-5 cells.
AB - Proteomic analyses of differentially expressed proteins in rat retinal ganglion cells (RGC-5) following S-nitrosoglutathione (GSNO), an NO donor, treatment were conducted. Of the approximately 314 protein spots that were detected, 19 were differentially expressed in response to treatment with GSNO. Of these, 14 proteins were up-regulated and 5 were down-regulated. Notably, an increase in GAPDH expression following GSNO treatment was detected in RGC-5 cells through Western blotting as well as proteomics. The increased GAPDH expression in response to GSNO treatment was accompanied by an increase in Herc6 protein, an E3 ubiquitin ligase. Moreover, GSNO treatment resulted in the translocation of GADPH from the cytosol to the nucleus and its subsequent accumulation. These results suggest that NO stress-induced apoptosis may be associated with the nuclear translocation and accumulation of GAPDH in RGC-5 cells.
KW - Glyceraldehyde-3-phosphate dehydrogenase
KW - NO
KW - Pro teomics
KW - Retinal ganglion cell (RGC-5)
UR - http://www.scopus.com/inward/record.url?scp=69449093180&partnerID=8YFLogxK
U2 - 10.5483/BMBRep.2009.42.7.456
DO - 10.5483/BMBRep.2009.42.7.456
M3 - Article
C2 - 19643045
AN - SCOPUS:69449093180
SN - 1976-6696
VL - 42
SP - 456
EP - 461
JO - BMB Reports
JF - BMB Reports
IS - 7
ER -