Rapid on-chip genetic detection microfluidic platform for real world applications

Satyajyoti Senapati, Andrew R. Mahon, Jason Gordon, Carsten Nowak, Shramik Sengupta, Thomas H.Q. Powell, Jeffrey Feder, David M. Lodge, Hsueh Chia Chang

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The development of genetic detection protocols for field applications is an important aspect of modern medical diagnostic technology and environmental monitoring. In this paper, we report a rapid, portable, and inexpensive DNA hybridization technique using a bead-based microfluidic platform that functions by passing fluorescently labeled target DNA through a chamber packed with functionalized beads within a microfluidic channel. DNA hybridization is then assessed using a digital camera attached to a Clare Chemical DR-45M dark reader non-UV transilluminator that uses visible light as an excitation source and a blue and amber filter to reveal fluorescence. This microfluidic approach significantly enhances hybridization by reducing the diffusion time between target DNA and the silica surface. The use of probe-functionalized beads as solid support also enhances the sensitivity and limit of detection due to a larger surface area per unit volume. This platform could be adapted for use in medical applications and environmental monitoring, including the detection of harmful organisms in the ballast water of ships.

Original languageEnglish
Article number022407
JournalBiomicrofluidics
Volume3
Issue number2
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Dive into the research topics of 'Rapid on-chip genetic detection microfluidic platform for real world applications'. Together they form a unique fingerprint.

Cite this