Regulation of cardiac malonyl-CoA content and fatty acid oxidation during increased cardiac power

Kristen L. King, Isidore C. Okere, Naveen Sharma, Jason R.B. Dyck, Aneta E. Reszko, Tracy A. McElfresh, Janos Kerner, Margaret P. Chandler, Gary D. Lopaschuk, William C. Stanley

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31 Scopus citations


Myocardial fatty acid oxidation is regulated by carnitine palmitoyltransferase I (CPT I), which is inhibited by malonyl-CoA. Increased cardiac power causes a fall in malonyl-CoA content and accelerated fatty acid oxidation; however, the mechanism for the decrease in malonyl-CoA is unclear. Malonyl-CoA is formed by acetyl-CoA carboxylase (ACC) and degraded by malonyl-CoA decarboxylase (MCD); thus a fall in malonyl-CoA could be due to activation of MCD, inhibition of ACC, or both. This study assessed the effects of increased cardiac power on malonyl-CoA content and ACC and MCD activities. Anesthetized pigs were studied under control conditions and during increased cardiac power in response to dobutamine infusion and aortic constriction alone, under hyperglycemic conditions, or with the CPT I inhibitor oxfenicine. An increase in cardiac power was accompanied by increased myocardial O2 consumption, decreased malonyl-CoA concentration, and increased fatty acid oxidation. There were no differences among groups in activity of ACC or AMP-activated protein kinase (AMPK), which physiologically inhibits ACC. There also were no differences in Vmax or Km of MCD. Previous studies have demonstrated that AMPK can be inhibited by protein kinase B (PKB); however, PKB was activated by dobutamine and the elevated insulin that accompanied hyperglycemia, but there was no effect on AMPK activity. In conclusion, the fall in malonyl-CoA and increase in fatty acid oxidization that occur with increased cardiac work were not due to inhibition of ACC or activation of MCD, suggesting alternative regulatory mechanisms for the work-induced decrease in malonyl-CoA concentration.

Original languageEnglish
Pages (from-to)H1033-H1037
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number3 58-3
StatePublished - Sep 2005


  • Acetyl coenzyme A carboxylase
  • Heart
  • Malonyl coenzyme A decarboxylase
  • Mitochondria
  • Protein kinase B


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