TY - JOUR
T1 - Remodeling of the endoplasmic reticulum in Caenorhabditis elegans oocytes is regulated by CGH-1
AU - Langerak, Shaughna
AU - Trombley, Alicia
AU - Patterson, Joseph R.
AU - Leroux, Devon
AU - Couch, Alexandra
AU - Wood, Megan P.
AU - Schisa, Jennifer A.
N1 - Funding Information:
The authors thank Dr. Jamie Alan and Dr. David Greenstein for reagents, Phil Oshel, Andrea Montalbano, and members of the Schisa lab for advice and support. Some strains were provided by the Caenorhabditis Genetics Center which is funded by NIH Office of research infrastructure programs, P40 OD010440. Funding for this work was provided in part by NIH 2R15GM109337 and NSF MRI1531277.
Funding Information:
National Science Foundation (NSF), Grant/ Award Number: MRI1531277; NIH, Grant/ Award Numbers: 2R15GM109337, P40 OD010440
Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2019/2
Y1 - 2019/2
N2 - A key aspect of development in all metazoans is remodeling at the cellular level. During the development of gametes, remodeling occurs throughout the germ line. When Caenorhabditis elegans hermaphrodites become depleted of sperm after 4 days of adulthood, significant cellular remodeling occurs within the meiotically-arrested oocytes, including the formation of ribonucleoprotein granules. Since major remodeling of the endoplasmic reticulum (ER) occurs in early embryos, we investigated the extent of ER remodeling in meiotically-arrested oocytes. We found, using a combination of fluorescence reporters and transmission electron microscopy, that the ER in arrested oocytes accumulates in patches and sheets that are enriched at the cortex. Our findings suggest this remodeling is not due to simple displacement by large amounts of yolk that accumulate in arrested oocytes, and instead may be genetically regulated. We further identified the Ddx6 RNA helicase, CGH-1, as a key regulator of ER in the germ line. In cgh-1(tn691) oocytes, we detected cortical ER patches as well as aberrant granules of the RNA-binding proteins, PAB-1, MEX-3, and CGH-1. Taken together, our results suggest the possibility that the spatial organization of RNA binding proteins may regulate the translation of mRNAs associated with the ER that in turn, controls the organization of the ER in the adult germ line.
AB - A key aspect of development in all metazoans is remodeling at the cellular level. During the development of gametes, remodeling occurs throughout the germ line. When Caenorhabditis elegans hermaphrodites become depleted of sperm after 4 days of adulthood, significant cellular remodeling occurs within the meiotically-arrested oocytes, including the formation of ribonucleoprotein granules. Since major remodeling of the endoplasmic reticulum (ER) occurs in early embryos, we investigated the extent of ER remodeling in meiotically-arrested oocytes. We found, using a combination of fluorescence reporters and transmission electron microscopy, that the ER in arrested oocytes accumulates in patches and sheets that are enriched at the cortex. Our findings suggest this remodeling is not due to simple displacement by large amounts of yolk that accumulate in arrested oocytes, and instead may be genetically regulated. We further identified the Ddx6 RNA helicase, CGH-1, as a key regulator of ER in the germ line. In cgh-1(tn691) oocytes, we detected cortical ER patches as well as aberrant granules of the RNA-binding proteins, PAB-1, MEX-3, and CGH-1. Taken together, our results suggest the possibility that the spatial organization of RNA binding proteins may regulate the translation of mRNAs associated with the ER that in turn, controls the organization of the ER in the adult germ line.
KW - early development
KW - nematode
KW - reproductive
UR - http://www.scopus.com/inward/record.url?scp=85058955670&partnerID=8YFLogxK
U2 - 10.1002/dvg.23267
DO - 10.1002/dvg.23267
M3 - Letter
C2 - 30489010
AN - SCOPUS:85058955670
VL - 57
JO - Genesis
JF - Genesis
SN - 1526-954X
IS - 2
M1 - e23267
ER -