Reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for zika virus and housekeeping genes in urine, serum, and mosquito samples

Sarah N. Bartolone, Maya O. Tree, Michael J. Conway, Michael B. Chancellor, Laura E. Lamb

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Infection with Zika virus (ZIKV) can be asymptomatic in adults, however, infection during pregnancy can lead to miscarriage and severe neurological birth defects. The goal of this protocol is to quickly detect ZIKV in both human and mosquito samples. The current gold standard for ZIKV detection is quantitative reverse transcription PCR (qRT-PCR); reverse transcription loop-mediated isothermal amplification (RT-LAMP) may allow for a more efficient and low-cost testing without the need for expensive equipment. In this study, RT-LAMP is used for ZIKV detection in various biological samples within 30 min, without first isolating the RNA from the sample. This technique is demonstrated using ZIKV infected patient urine and serum, and infected mosquito samples. 18S ribosomal ribonucleic acid and actin are used as controls in human and mosquito samples, respectively.

Original languageEnglish
Article numbere58436
JournalJournal of Visualized Experiments
Volume2018
Issue number139
DOIs
StatePublished - Sep 14 2018

Keywords

  • Aedes
  • Field tools
  • Genetics
  • Issue 139
  • Point-of-care testing
  • RT-LAMP
  • Serum
  • Urine
  • Zika virus

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