Single Molecule Fluorescence Correlation Spectroscopy in an Electrophoretic Mobility Shift Assay

Dale J. LeCaptain, Alan Van Orden

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A capillary electrophoresis-based electrophoretic mobility shift assay is described wherein fluorescence correlation spectroscopy is used to resolve the bound and unbound fractions of a DNA-protein complex on the basis of their characteristic electrophoretic flow velocities.

Original languageEnglish
Title of host publicationLaser Applications to Chemical, Security and Environmental Analysis, LACSEA 2002
PublisherOptica Publishing Group (formerly OSA)
ISBN (Electronic)1557526990
StatePublished - 2002
EventLaser Applications to Chemical, Security and Environmental Analysis, LACSEA 2002 - Boulder, United States
Duration: Feb 7 2002 → …

Publication series

NameOptics InfoBase Conference Papers

Conference

ConferenceLaser Applications to Chemical, Security and Environmental Analysis, LACSEA 2002
Country/TerritoryUnited States
CityBoulder
Period02/7/02 → …

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