TY - JOUR
T1 - Soluble factors from neuronal cultures induce a specific proliferation and resistance to apoptosis of cognate mouse skeletal muscle precursor cells
AU - Pelletier, Maude
AU - Rossignol, Julien
AU - Oliver, Lisa
AU - Zampieri, Maryvonne
AU - Fontaine-Pérus, Josiane
AU - Vallette, François M.
AU - Lescaudron, Laurent
N1 - Funding Information:
MP was a recipient of a fellowship from the Association pour la Recherche contre le Cancer (ARC). This work was supported by INSERM and the University of Nantes (F.M.V., L.L.), by CNRS and Association Française contre les Myopathies grants to J.F.-P.
PY - 2006/10/16
Y1 - 2006/10/16
N2 - The mechanisms or the physiological events, which control the regeneration of skeletal muscle through muscle precursor cell multiplication and differentiation, are still largely unknown. To address the question of the involvement of neurons in this process, skeletal muscle progenitors were grown in the presence of conditioned media obtained from 3-day-old cultures of embryonic neurons (derived from either the dorsal or the ventral region of 11-day-old mouse embryos) or media conditioned with satellite cells. Strikingly, only satellite cells cultured in medium conditioned from ventral embryonic neurons exhibited increased proliferation, as well as resistance to staurosporine (STS)-induced apoptosis. Our results suggest the existence of specific anti-apoptogenic neural soluble signals, which could be involved in skeletal muscle regeneration pathways.
AB - The mechanisms or the physiological events, which control the regeneration of skeletal muscle through muscle precursor cell multiplication and differentiation, are still largely unknown. To address the question of the involvement of neurons in this process, skeletal muscle progenitors were grown in the presence of conditioned media obtained from 3-day-old cultures of embryonic neurons (derived from either the dorsal or the ventral region of 11-day-old mouse embryos) or media conditioned with satellite cells. Strikingly, only satellite cells cultured in medium conditioned from ventral embryonic neurons exhibited increased proliferation, as well as resistance to staurosporine (STS)-induced apoptosis. Our results suggest the existence of specific anti-apoptogenic neural soluble signals, which could be involved in skeletal muscle regeneration pathways.
KW - Expansion
KW - Muscle precursor cells
KW - Neuron
KW - Survival
UR - http://www.scopus.com/inward/record.url?scp=33748428942&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2006.06.076
DO - 10.1016/j.neulet.2006.06.076
M3 - Article
C2 - 16959418
AN - SCOPUS:33748428942
VL - 407
SP - 20
EP - 25
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -