Sortase-catalyzed peptide-glycosylphosphatidylinositol analogue ligation

Xueqing Guo, Qianli Wang, Benjamin M. Swarts, Zhongwu Guo

Research output: Contribution to journalArticlepeer-review

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(Figure Presented) It is demonstrated that sortase A (SrtA) can catalyze efficient coupling of peptides to GPI analogues with a glycine residue attached to the phosphoethanolamine moiety at the nonreducing end to form GPI-linked peptides. This represents the first chemoenzymatic synthesis of GPI-peptide conjugates and is a proof-of-concept for the potential application of SrtA to the synthesis of more complex GPI-anchored peptides/glycopeptides and GPI-anchored proteins/glycoproteins.

Original languageEnglish
Pages (from-to)9878-9879
Number of pages2
JournalJournal of the American Chemical Society
Issue number29
StatePublished - Jul 29 2009


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