Abstract
1. 1. A series of tests were conducted to determine whether or not the hemolymph of the crayfish, Orconectes virilis, contains a plasma coagulation factor analogous to mammalian fibrinogen. 2. 2. Anti-coagulated hemolymph free from cell material could not be induced to clot by the addition of calcium chloride, whereas clot formation proceeded when whole cells and/or cell debris was present. 3. 3. Thrombin and rabbit anti-human and anti-bovine fibrinogen antisera failed to react with any crayfish samples used. 4. 4. All hemolymph samples exhibited similar electrophoretic banding patterns. 5. 5. Prothrombin and partial thromboplastin time tests using human reagents yielded negative results with all hemolymph samples studied.
| Original language | English |
|---|---|
| Pages (from-to) | 41-44,IN5-IN6,45-48 |
| Journal | Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
| Volume | 42 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 15 1972 |
Keywords
- Crustacea
- Orconectes virilis
- Plasma coagulation factors
- cellulose acetate electrophoresis
- clotting protein
- coagulation
- crayfish
- crayfish hemolymph
- crustacean hemolymph
- dextran gel
- disc electrophoresis
- hemolymph coagulation
- interfacial precipitin
- partial thromboplastin
- prothrombin