Synthesis of a low thrombogenic heart valve coating with horseradish peroxidase

Adrienne M. Benson, Kamel Omrane, Erin M. Graves, Amy L. Haas, Erica Bryant, Monisha Mandalaywala, Anja Mueller

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cardiovascular implants like artificial heart valves are made out of a variety of materials to allow for adequate mechanical properties and mechanical movement. All of these materials have different adhesion properties towards blood proteins but all still induce blood coagulation. This article describes the design and enzymatic synthesis of a heart valve coating that would adhere to different materials as well as prevent adhesion of proteins to the surface to prevent blood coagulation. It consists of two covalently linked hyperbranched polymer thin films: one that provides strong adhesion to the surface in question and another that prevents adhesion of blood proteins. The synthesis of hyperbranched polyresorcinol and linear polyhydroquinone is presented for the thin film that adheres to the artificial heart valve. The synthesis of polyglycerol and poly(glucuronic acid) is presented for the anti-thrombogenic thin film. The polymers are synthesized by horseradish peroxidase in water or buffer. Some of these syntheses do not use any other oxidant than oxygen, and the mechanism of these reactions is currently under investigation. The syntheses still need to be characterized in more detail and optimized. These polymers will be covalently linked to form the heart valve coating.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalPolymers for Advanced Technologies
Volume16
Issue number2-3
DOIs
StatePublished - Feb 2005
Externally publishedYes

Keywords

  • Biomaterials
  • Enzymatic polymer synthesis
  • Heart valve coating
  • Hyperbranched
  • Polyethers

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