TY - JOUR
T1 - Tart cherry extract and omega fatty acids reduce behavioral deficits, gliosis, and amyloid-beta deposition in the 5xfad mouse model of alzheimer’s disease
AU - Bowers, Zackary
AU - Maiti, Panchanan
AU - Bourcier, Ali
AU - Morse, Jarod
AU - Jenrow, Kenneth
AU - Rossignol, Julien
AU - Dunbar, Gary L.
N1 - Funding Information:
Funding: Support for this study was provided by InTerra Nutraceuticals, Inc., the Field Neurosciences Institute, and the John G. Kulhavi Professorship in Neuroscience at CMU.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - Combined treatments using polyphenols and omega fatty acids provide several therapeutic benefits for a variety of age-related disorders, including Alzheimer’s disease (AD). Previously, we found a commercial product, Total Body Rhythm (TBR), consisting of tart cherry extract, a potent polyphenol, and omega fatty acids, significantly reduced memory, and neuropathological deficits in the 192 IgG-saporin mouse model of AD. The present study assessed the efficacy of TBR for treating behavioral and neuropathological deficits in the 5xFAD model of AD. Both 6-and 12-month-old 5xFAD mice and age-matched wild-type controls received TBR (60 mg/kg) or the equivalent dose of vehicle (0.5% methylcellulose) via oral administration, every other day for two months. All mice were tested in the open field (OF), novel object recognition (NOR), and the Morris water maze (MWM) tasks. In addition, neuronal morphology, neurodegeneration, Aβ plaque load, and glial activation were assessed. TBR treatment reduced memory deficits in the MWM and NOR tests and lessened anxiety levels in the OF task, mostly in the 6-month-old male mice. TBR also protected against neuron loss, reduced activation of astrocytes and microglia, primarily in 6-month-old mice, and attenuated Aβ deposition. These results suggest that the combination of tart cherry extract and omega fatty acids in TBR can reduce AD-like deficits in 5xFAD mice.
AB - Combined treatments using polyphenols and omega fatty acids provide several therapeutic benefits for a variety of age-related disorders, including Alzheimer’s disease (AD). Previously, we found a commercial product, Total Body Rhythm (TBR), consisting of tart cherry extract, a potent polyphenol, and omega fatty acids, significantly reduced memory, and neuropathological deficits in the 192 IgG-saporin mouse model of AD. The present study assessed the efficacy of TBR for treating behavioral and neuropathological deficits in the 5xFAD model of AD. Both 6-and 12-month-old 5xFAD mice and age-matched wild-type controls received TBR (60 mg/kg) or the equivalent dose of vehicle (0.5% methylcellulose) via oral administration, every other day for two months. All mice were tested in the open field (OF), novel object recognition (NOR), and the Morris water maze (MWM) tasks. In addition, neuronal morphology, neurodegeneration, Aβ plaque load, and glial activation were assessed. TBR treatment reduced memory deficits in the MWM and NOR tests and lessened anxiety levels in the OF task, mostly in the 6-month-old male mice. TBR also protected against neuron loss, reduced activation of astrocytes and microglia, primarily in 6-month-old mice, and attenuated Aβ deposition. These results suggest that the combination of tart cherry extract and omega fatty acids in TBR can reduce AD-like deficits in 5xFAD mice.
KW - 5xFAD
KW - Alzheimer’s disease
KW - Antioxidants
KW - Inflammation
KW - Neurodegeneration
KW - Omega fatty acids
KW - Total body rhythm
UR - http://www.scopus.com/inward/record.url?scp=85118273455&partnerID=8YFLogxK
U2 - 10.3390/brainsci11111423
DO - 10.3390/brainsci11111423
M3 - Article
AN - SCOPUS:85118273455
VL - 11
JO - Brain Sciences
JF - Brain Sciences
SN - 2076-3425
IS - 11
M1 - 1423
ER -