The late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis

Philip L. Hertzler; Wallis H. Clark

doi:10.1017/S0967199400001611

The late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis

Philip L. Hertzler, Wallis H. Clark

Biology

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1 Scopus citations

Abstract

Antibodies to sea urchin β-tubulin and mammalian heavy neurofilaments were used to study the late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis. The neurofilament antibody fortuitously stained centrosomes in eggs, as well as the subacrosomal region and acrosomal filament in sperm. The neurofilament antibody also stained a cortial site in eggs which was associated with the positioning of the mitotic spindle. During pronuclear migration, a large maternal microtubule aster formed in addition to the sperm aster. The activity of the maternal centrosome disappeared during syngamy, while the sperm centrosomes formed the poles of the first mitotic spindle. Colcemid treatment modulated the size of the mitotic spindle and blocked pronuclear migration.

Original language	English
Pages (from-to)	287-296
Number of pages	10
Journal	Zygote
Volume	1
Issue number	4
DOIs	https://doi.org/10.1017/S0967199400001611
State	Published - Nov 1993

Keywords

Centrosomes
Fertilisation
Intermediate filaments
Microtubules
Penaeoidean shrimp

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10.1017/S0967199400001611

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title = "The late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis",

abstract = "Antibodies to sea urchin β-tubulin and mammalian heavy neurofilaments were used to study the late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis. The neurofilament antibody fortuitously stained centrosomes in eggs, as well as the subacrosomal region and acrosomal filament in sperm. The neurofilament antibody also stained a cortial site in eggs which was associated with the positioning of the mitotic spindle. During pronuclear migration, a large maternal microtubule aster formed in addition to the sperm aster. The activity of the maternal centrosome disappeared during syngamy, while the sperm centrosomes formed the poles of the first mitotic spindle. Colcemid treatment modulated the size of the mitotic spindle and blocked pronuclear migration.",

keywords = "Centrosomes, Fertilisation, Intermediate filaments, Microtubules, Penaeoidean shrimp",

author = "Hertzler, {Philip L.} and Clark, {Wallis H.}",

note = "Funding Information: The authors thank Dr Roger Leslie for the gift of antibodies and Dr Robert Summers for advice on confocal microscopy. This paper was funded by California Sea Grant NA89AA-D-SG140, United States Department of Agriculture Grant 87 CRCR-1-2514, and NIH predoctoral fellowship HD07131 (P.L.H.).",

year = "1993",

month = nov,

doi = "10.1017/S0967199400001611",

language = "English",

volume = "1",

pages = "287--296",

journal = "Zygote",

issn = "0967-1994",

number = "4",

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TY - JOUR

T1 - The late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis

AU - Hertzler, Philip L.

AU - Clark, Wallis H.

N1 - Funding Information: The authors thank Dr Roger Leslie for the gift of antibodies and Dr Robert Summers for advice on confocal microscopy. This paper was funded by California Sea Grant NA89AA-D-SG140, United States Department of Agriculture Grant 87 CRCR-1-2514, and NIH predoctoral fellowship HD07131 (P.L.H.).

PY - 1993/11

Y1 - 1993/11

N2 - Antibodies to sea urchin β-tubulin and mammalian heavy neurofilaments were used to study the late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis. The neurofilament antibody fortuitously stained centrosomes in eggs, as well as the subacrosomal region and acrosomal filament in sperm. The neurofilament antibody also stained a cortial site in eggs which was associated with the positioning of the mitotic spindle. During pronuclear migration, a large maternal microtubule aster formed in addition to the sperm aster. The activity of the maternal centrosome disappeared during syngamy, while the sperm centrosomes formed the poles of the first mitotic spindle. Colcemid treatment modulated the size of the mitotic spindle and blocked pronuclear migration.

AB - Antibodies to sea urchin β-tubulin and mammalian heavy neurofilaments were used to study the late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis. The neurofilament antibody fortuitously stained centrosomes in eggs, as well as the subacrosomal region and acrosomal filament in sperm. The neurofilament antibody also stained a cortial site in eggs which was associated with the positioning of the mitotic spindle. During pronuclear migration, a large maternal microtubule aster formed in addition to the sperm aster. The activity of the maternal centrosome disappeared during syngamy, while the sperm centrosomes formed the poles of the first mitotic spindle. Colcemid treatment modulated the size of the mitotic spindle and blocked pronuclear migration.

KW - Centrosomes

KW - Fertilisation

KW - Intermediate filaments

KW - Microtubules

KW - Penaeoidean shrimp

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U2 - 10.1017/S0967199400001611

DO - 10.1017/S0967199400001611

M3 - Article

C2 - 8081826

AN - SCOPUS:0027688668

SN - 0967-1994

VL - 1

SP - 287

EP - 296

JO - Zygote

JF - Zygote

IS - 4

ER -

The late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis

Abstract

Keywords

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