TY - JOUR
T1 - The turbid specimen as an analytical medium
T2 - hemoglobin determination as a model
AU - Sharma, Ajit
AU - Artiss, Joseph D.
AU - Strandbergh, Donald R.
AU - Zak, Bennie
PY - 1985/3/30
Y1 - 1985/3/30
N2 - The quantitation of chemical constituents in lipemic samples is a major problem confronting the clinical laboratory. Currently, a number of cumbersome and timeconsuming methods are used to clarify samples before analysis. However, the use of enzymic hydrolysis of triglycerides along with efficient chemical removal of the formed non-esterified fatty acids is exemplified here as an excellent alternative to the current methods of clarification such as ultracentrifugation, extraction or chemical precipitation of low density and very low density lipoproteins. This method of clarifying milky serum has been used by us to assay hemoglobin in severely lipemic blood samples as an analytical model.
AB - The quantitation of chemical constituents in lipemic samples is a major problem confronting the clinical laboratory. Currently, a number of cumbersome and timeconsuming methods are used to clarify samples before analysis. However, the use of enzymic hydrolysis of triglycerides along with efficient chemical removal of the formed non-esterified fatty acids is exemplified here as an excellent alternative to the current methods of clarification such as ultracentrifugation, extraction or chemical precipitation of low density and very low density lipoproteins. This method of clarifying milky serum has been used by us to assay hemoglobin in severely lipemic blood samples as an analytical model.
KW - Hemoglobin determination
KW - Lipase
KW - Lipemic sample
KW - α-Cyclodextrin
UR - http://www.scopus.com/inward/record.url?scp=0021921405&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(85)90004-X
DO - 10.1016/0009-8981(85)90004-X
M3 - Article
C2 - 3987056
AN - SCOPUS:0021921405
SN - 0009-8981
VL - 147
SP - 7
EP - 14
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1
ER -