TY - JOUR
T1 - Tissue plasminogen activator induced fibrinolysis
T2 - Standardization of method using thromboelastography
AU - Kupesiz, Alphan
AU - Rajpurkar, Madhvi
AU - Warrier, Indira
AU - Hollon, Wendy
AU - Tosun, Ozgur
AU - Lusher, Jeanne
AU - Chitlur, Meera
PY - 2010/6
Y1 - 2010/6
N2 - Fibrinolysis is a complex physiological process that involves the interaction of several anticoagulant proteins. Defects of the fibrinolytic system are extremely difficult to diagnose and study because there are no standardized tests available. Thromboelastography is a novel method that allows the study of both coagulation and fibrinolysis using one sample of whole blood, thereby allowing a more physiologic assessment of the coagulation process. Several in-vitro studies have been attempted to determine whether thromboelastography would be a useful assay for the study of fibrinolysis but have reported problems with reproducibility and reliability. Here we report the process involved in developing a thromboelastographic assay in which tissue plasminogen activator (t-PA) is used to induce fibrinolysis. The assay was standardized to ensure that the concentration of the coagulation inducer (tissue factor) and fibrinolytic agent (t-PA) was adequate to induce a clot with lysis parameters that were reproducible and reliable. This method can be used to rapidly assess the intrinsic fibrinolytic potential of whole blood. Our assay showed that it could rapidly predict high levels of plasminogen activator inhibitor, and this information would be beneficial in patients with acute stroke or myocardial infarction.
AB - Fibrinolysis is a complex physiological process that involves the interaction of several anticoagulant proteins. Defects of the fibrinolytic system are extremely difficult to diagnose and study because there are no standardized tests available. Thromboelastography is a novel method that allows the study of both coagulation and fibrinolysis using one sample of whole blood, thereby allowing a more physiologic assessment of the coagulation process. Several in-vitro studies have been attempted to determine whether thromboelastography would be a useful assay for the study of fibrinolysis but have reported problems with reproducibility and reliability. Here we report the process involved in developing a thromboelastographic assay in which tissue plasminogen activator (t-PA) is used to induce fibrinolysis. The assay was standardized to ensure that the concentration of the coagulation inducer (tissue factor) and fibrinolytic agent (t-PA) was adequate to induce a clot with lysis parameters that were reproducible and reliable. This method can be used to rapidly assess the intrinsic fibrinolytic potential of whole blood. Our assay showed that it could rapidly predict high levels of plasminogen activator inhibitor, and this information would be beneficial in patients with acute stroke or myocardial infarction.
KW - Fibrinolysis
KW - Plasminogen activator inhibitor
KW - Thromboelastography
KW - Tissue factor
KW - Tissue plasminogen activator
UR - http://www.scopus.com/inward/record.url?scp=77951906209&partnerID=8YFLogxK
U2 - 10.1097/MBC.0b013e32833464e9
DO - 10.1097/MBC.0b013e32833464e9
M3 - Article
C2 - 20400895
AN - SCOPUS:77951906209
VL - 21
SP - 320
EP - 324
JO - Blood Coagulation and Fibrinolysis
JF - Blood Coagulation and Fibrinolysis
SN - 0957-5235
IS - 4
ER -