TY - JOUR
T1 - Water soluble cationic dextran derivatives containing poly(amidoamine) dendrons for efficient gene delivery
AU - Mai, Kaijin
AU - Zhang, Shanshan
AU - Liang, Bing
AU - Gao, Cong
AU - Du, Wenjun
AU - Zhang, Li Ming
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China ( 51273216 , 21074152 , 81100890 ), Doctoral Research Program of Education Ministry in China ( 20090171110023 and 20130171120095 ), Key Project of Scientific and Technical Innovation for Universities in Guangdong Province ( cxzd1102 ) and Natural Science Foundation of Guangdong Province ( S2013010012549 and S2013040014401 ).
Publisher Copyright:
©2015 Elsevier Ltd. All rights reserved.
PY - 2015/6/5
Y1 - 2015/6/5
N2 - To develop new dextran derivatives for efficient gene delivery, the conjugation of poly(amidoamine) dendrons onto biocompatible dextran was carried out by a Cu(I)-catalyzed azide-alkyne cycloaddition, as confirmed by FTIR and 1H NMR analyses. For resultant dextran conjugates with various generations of poly(amidoamine) dendrons, their buffering capacity and in vitro cytotoxicity were evaluated by acid-base titration and MTT tests, respectively. In particular, their physicochemical characteristics for the complexation with plasmid DNA were investigated by the combined analyses from agarose gel electrophoresis, zeta potential, particle size, transmission electron microscopy and fluorescence emission spectra. Moreover, their complexes with plasmid DNA were studied with respect to their transfection efficiency in human embryonic kidney (HEK293) cell lines. In the case of a higher generation of poly(amidoamine) dendrons, such a dextran conjugate was found to have much lower cytotoxicity and better gene delivery capability when compared to branched polyethylenimine (bPEI, 25 kDa), a commonly used gene vector.
AB - To develop new dextran derivatives for efficient gene delivery, the conjugation of poly(amidoamine) dendrons onto biocompatible dextran was carried out by a Cu(I)-catalyzed azide-alkyne cycloaddition, as confirmed by FTIR and 1H NMR analyses. For resultant dextran conjugates with various generations of poly(amidoamine) dendrons, their buffering capacity and in vitro cytotoxicity were evaluated by acid-base titration and MTT tests, respectively. In particular, their physicochemical characteristics for the complexation with plasmid DNA were investigated by the combined analyses from agarose gel electrophoresis, zeta potential, particle size, transmission electron microscopy and fluorescence emission spectra. Moreover, their complexes with plasmid DNA were studied with respect to their transfection efficiency in human embryonic kidney (HEK293) cell lines. In the case of a higher generation of poly(amidoamine) dendrons, such a dextran conjugate was found to have much lower cytotoxicity and better gene delivery capability when compared to branched polyethylenimine (bPEI, 25 kDa), a commonly used gene vector.
KW - Click conjugatation
KW - DNA Gene delivery
KW - Dextran conjugate
KW - Plasmid
KW - Poly(amidoamine) dendrons
UR - http://www.scopus.com/inward/record.url?scp=84922801893&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2015.01.042
DO - 10.1016/j.carbpol.2015.01.042
M3 - Article
C2 - 25843855
AN - SCOPUS:84922801893
SN - 0144-8617
VL - 123
SP - 237
EP - 245
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -