Water soluble cationic dextran derivatives containing poly(amidoamine) dendrons for efficient gene delivery

Kaijin Mai, Shanshan Zhang, Bing Liang, Cong Gao, Wenjun Du, Li Ming Zhang

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

To develop new dextran derivatives for efficient gene delivery, the conjugation of poly(amidoamine) dendrons onto biocompatible dextran was carried out by a Cu(I)-catalyzed azide-alkyne cycloaddition, as confirmed by FTIR and 1H NMR analyses. For resultant dextran conjugates with various generations of poly(amidoamine) dendrons, their buffering capacity and in vitro cytotoxicity were evaluated by acid-base titration and MTT tests, respectively. In particular, their physicochemical characteristics for the complexation with plasmid DNA were investigated by the combined analyses from agarose gel electrophoresis, zeta potential, particle size, transmission electron microscopy and fluorescence emission spectra. Moreover, their complexes with plasmid DNA were studied with respect to their transfection efficiency in human embryonic kidney (HEK293) cell lines. In the case of a higher generation of poly(amidoamine) dendrons, such a dextran conjugate was found to have much lower cytotoxicity and better gene delivery capability when compared to branched polyethylenimine (bPEI, 25 kDa), a commonly used gene vector.

Original languageEnglish
Pages (from-to)237-245
Number of pages9
JournalCarbohydrate Polymers
Volume123
DOIs
StatePublished - Jun 5 2015

Keywords

  • Click conjugatation
  • DNA Gene delivery
  • Dextran conjugate
  • Plasmid
  • Poly(amidoamine) dendrons

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