West Nile virus infection alters midgut gene expression in Culex pipiens quinquefasciatus say (Diptera: Culicidae)

Chelsea T. Smartt, Stephanie L. Richards, Sheri L. Anderson, Jennifer S. Erickson

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Alterations in gene expression in the midgut of female Culex pipiens quinquefasciatus exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding on infected blood. Of these, 21 cDNAs showed an increase in expression, and 5 showed a decrease in expression as a result of WNV presence in the blood meal. GenBank database searches showed that one clone with increased expression, CQ G12A2, shares 94% identity with a leucine-rich repeat-containing protein from Cx. p. quinquefasciatus and 32% identity to Toll-like receptors from Aedes aegypti. We present the first cDNA clone isolated from female Cx. p. quinquefasciatus midgut tissue whose expression changes on exposure to WNV. This cDNA represents a mosquito gene that is an excellent candidate for interacting with WNV in Cx. p. quinquefasciatus and may play a role in disease transmission.

Original languageEnglish
Pages (from-to)258-263
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume81
Issue number2
DOIs
StatePublished - Aug 2009
Externally publishedYes

Fingerprint

Dive into the research topics of 'West Nile virus infection alters midgut gene expression in Culex pipiens quinquefasciatus say (Diptera: Culicidae)'. Together they form a unique fingerprint.

Cite this